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Recognizes the ~53 kDa mutant p53 protein under non-denaturing conditions by immunoprecipitation, immunofluorescence, and flow cytometry. Recognizes both mutant and wild-type p53 by immunoblotting and paraffin sections under denaturing conditions.
| Acessórios para Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) |
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Informações sobre o produto
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Host
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Mouse
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Isotype
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IgG1
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Immunogen
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a recombinant protein consisting of amino acids 14-389 of p53 fused to β-galactosidase
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Epitope
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within amino acids 213-217
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Clone
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PAb240
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Form
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Liquid
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Formulation
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In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
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Species reactivity
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bovine, chicken, hamster, human, mouse, rat, not Xenopus
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Preservative
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≤0.1% sodium azide
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Applications
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Immunoprecipitation
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Gel Shift
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Immunofluorescence
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Flow Cytometry
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Frozen Sections
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Immunoblotting (Western Blotting)
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Paraffin Sections
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Do not freeze
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Sim
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Positive control
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A431, Hs27 (wild-type p53), or SK-BR-3 cells or breast carcinoma tissue
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Negative control
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SK-OV-3 cells
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Informação de estoque e transporte
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Armazenagem
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+2°C to +8°C
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Ship
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Blue or Dry Ice
Standard Handling
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