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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Telefone: +49 6151 72-0
Fax: +49 6151 72 2000
31 Julho 2010
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| Nº de produto | Tamanho |
|---|---|
| PC35-1EA | 1 ea |
Recognizes the ~53 kDa wild-type and mutant p53 protein in A431 and SVT2 cells and breast carcinoma tissue.
| Informações sobre o produto | |||
|---|---|---|---|
| Host | Sheep | ||
| Isotype | IgG | ||
| Immunogen | recombinant, human p53 protein | ||
| Form | Liquid | ||
| Formulation | 200 µl diluted (1:4) anti-p53 sheep serum, 500 µl undiluted normal sheep serum, and 100 µl biotinylated rabbit anti-sheep IgG. | ||
| Species reactivity | human, mouse, rat | ||
| Preservative | ≤0.1% sodium azide (Anti-p53 and Anti-Sheep IgG only) | ||
| Applications |
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| Positive control | A431 or SVT2 cells or breast carcinoma tissue | ||
| Kit contains | Anti-p53 antisera, normal sheep serum, and biotinylated rabbit anti-sheep IgG | ||
| Informação de estoque e transporte | |||
|---|---|---|---|
| Armazenagem | -20°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Dados | |||
|---|---|---|---|
 Detection of mouse p53 by immunoblotting. Sample: Whole cell lysate (10 μg) from SVT2 cells. Primary antibody: Anti-p53 (Ab-7) (Pantropic) Sheep pAb (Cat. No. PC35) (1:2500) (lane 1) or normal sheep serum (lane 2). Detection: chemiluminescence. |
